Mir-23b down-regulates the expression of target gene of acetaldehyde dehydrogenase 1a1 and increases the sensitivity of cervical cancer stem cells to cisplatin

• DOI: 10.4314/tjpr.v20i1.5
• Publication Date: 2022-01-14T09:16:26Z
• Authors: Yongbing Tao; Fuyun Mao; Weihong Gu; Ling Wu; Jing Guo; Ping Xu
• Keywords: HeLa; clone (Java method); Growth inhibition

Purpose: To study the effect of miR-23b on expression target gene acetaldehyde dehydrogenase 1A1 (ALDH1A1), and cisplatin (CDDP) susceptibility cervical carcinoma stem cells.
 Methods: Human cancer cell line Hela cells were cultured in vitro, mimic negative control transfected into using lipofectamine method. The growth two groups was determined curve method, their proliferation measured plate clone formation. influence treatments sensitivity to CDDP assayed MTT mRNA ALDH1A1 real-time quantitative polymerase hain reation (PCR), while its protein by Western blot.
 Results: levels expressions overexpression group significantly lower than those (p < 0.05). sensitivities inhibition dose-dependent some extent, but markedly increased, relative when dose 0.1 ppc 0.01).
 Conclusion: Up-regulating inhibits cells, increases via down-regulation for ALDH1A1. Therefore, during treatment, increasing level may produce a chemotherapeutic effect.
 Keywords: MiR-23b, Acetaldehyde 1A1, Cervical cancer, Cisplatin, Sensitivity