Isolation and characterization of virulent bacteriophages against Klebsiella pneumoniae of significant capsular types

• DOI: 10.47183/mes.2023.060
• Publication Date: 2024-01-22T14:30:39Z
• Authors: RB Gorodnichev; MA Kornienko; DA Bespiatykh; MV Malakhova; AO Krivulia; VA Veselovsky; JA Bespyatykh; OV Goloshchapov; TV Chernenkaya; EA Shitikov
• Keywords: Lytic cycle; Phage therapy; Myoviridae; Lysogenic cycle

The growing proportion of antibiotic-resistant Klebsiella pneumoniae strains raises challenges to the healthcare system and requires development alternative treatment options. Bacteriophage therapy is one such study was aimed isolate describe bacteriophages effective against K. clinically significant capsular types. were isolated from sewage river water samples using enrichment culture technique. spectrum lytic activity phages tested on collection clinical isolates (n = 279). studied lysed 52.8–100% respective types: phage VKV295 100% with type KL1, SAA231 — 52.8 KL2, NNK-G4 KL39, VSG32 66.7% KL41, NKA196 87.5% KL47, Rappa3 KL57, PEA128 95.5% KL64, ChM-G5 69.6% KL102. Whole-genome sequencing subsequent bioinformatic analysis revealed that belong Autographiviridae family are classified into three genera.The limited specific types due presence receptor-binding proteins, polysaccharide depolymerases. strictly virulent, did not carry harmful genetic determinants, had a host range, making them applicable in therapeutic practice for combating infections caused by pneumoniae.