Studying the Innate Immune Response to Myocardial Infarction in a Highly Efficient Experimental Animal Model

• DOI: 10.47803/rjc.2021.31.3.573
• Publication Date: 2021-10-12T10:07:21Z
• Authors: Razvan Gheorghita...; Doina Manu; Istvan Adorjan Szabo; Mihaela Elena Tomut; Gabriela Pintican; Bogdan Cordos; Gabriel Jakobsson; Minodora Dobreanu; Ovidiu Simion Cotoi; Alexandru Schiopu
• Keywords: MERTK

Abstract The reduction in mortality following acute myocardial infarction (AMI) is an important achievement of modern medicine. Despite this progress, AMI remains the most common cause heart failure (HF) and HF-related morbidity mortality. involvement innate immune response different stages after has at tracted attention recent years. With increasing range potential therapeutic compounds delivery vectors, need highly efficient experimental models increasing, to support further advancement field. Here, we present a high-throughput model for assessment AMI. based on permanent surgical ligation left descending coronary artery (LAD) mice, followed by complex flow-cytometry histological analyses cellular populations blood myocardium. We are presenting time-dependent qualitative quantitative analysis results, demonstrating intense accumulation Ly6G hi neutrophils Ly6C monocytes infarcted myocardium days 1 3 post-AMI, successive reparatory lo MerTK macrophages, neovascularization fibrosis development day 7.