Steroids produced by Cladosporium anthropophilum, an endophytic fungus Isolated from Avicennia marina (Forssk.) Vierh and Their Antibacterial Activity

• DOI: 10.48048/tis.2024.8498
• Publication Date: 2024-10-25T16:46:48Z
• Authors: Yeni Mulyani; Al Arofatus Naini; Kindi Farabi; Fajar Fauzi Abdullah; Wahyu Safriansyah; Siska Elisahbet S...; Azmi Azhari; Sari Purbaya; Asri Peni Wulandari; Sofa Fajriah; Yoshihito Shiono; Unang Supratman
• Keywords: Avicennia marina; Cladosporium

This work was conducted to isolate the steroid compounds from an endophytic fungus Cladosporium anthropophilum and determine their effects against 4 strains of bacteria. The fungi were afforded a mangrove plant Avicennia marina (Forssk.) Vierh Acanthaceae family, growing in West Java, Indonesia. fermentation isolation C. yielded 3 ergostane-type steroids including highly oxidized one, identified as penicisteroid A (1), ergosterol (2), ergosterol-5,8-peroxide (3), together with 2 stigmastanes, characterized stigmasterol (4), stigmasterol-5,8-peroxide (5). elucidation structure all isolated performed by extensive spectroscopic measurements (MS, IR, 1D, 2D NMR) supported comparison previously reported spectral data. Subsequently, potential 5 well methanol, n-hexane, ethyl acetate extracts evaluated Gram-positive bacteria, Staphylococcus aureus ATCC 29213 Enterococcus faecalis 29212 Gram-negative Vibrio harveyi 5339 Escherichia coli 25922. results showed that none active, while exhibited significant inhibition tested bacteria’s growth enabled us propose synergistic between active these antibacterial activity. HIGHLIGHTS An fungus, anthropophilum, five steroids. chromatography technique method utilized for separation processes determination, respectively. assay two bacteria ( E. faecalis) (V. coli) implied parent extracts. further investigation is recommended explore possibility source compounds. GRAPHICAL ABSTRACT