Interaction of Epithelial Cells with Surfaces and Surfaces Decorated by Molecules

• DOI: 10.48550/arxiv.1303.0392
• Publication Date: 2013-01-01
• Authors: Daniele De Martini; Othmar Marti; Michael Beil; Tobias Paust; Chenyu Huang; Markus Moosmann; Jiyu Jin; Tobias Heiler; Roland Gröger; Thomas Schimmel; Stefan Walheim
• Keywords: Nanometre

A detailed understanding of the interface between living cells and substrate materials is rising importance in many fields medicine, biology biotechnology. Cells at interfaces often form epithelia. The physical barrier that they one their main functions. It governed by properties networks forming cytoskeleton systems cell-to-cell contacts. Different substrates with varying surface modify migration velocity cells. On hand can change composition. Organic inorganic induce differing velocities same cell system. Within class materials, a stiffness or energy modifies velocity, too. For our adhesion studies variety different, homogeneous were used (polymers, bio-polymers, metals, oxides). In addition, an effective lithographic method, Polymer Blend Lithography (PBL), reported, to produce patterned Self-Assembled Monolayers (SAM) on solid featuring two three different chemical functionalities. This we achieve without use conventional lithography like e-beam UV lithography, only using self-organization. These surfaces are decorated Teflon-like amino-functionalized molecular layer. resulting pattern copy previously created self-organized polymer pattern, scalable lateral domain size sub-micron range down below 100 nanometers. monolayer features high biofunctional contrast feature sizes complexes e.g. focal points.