smFRET reveals DHX36 repetitive binding,not unfolding,of G-quadruplexes

• DOI: 10.48550/arxiv.2106.07858
• Publication Date: 2021-01-01
• Authors: Hai‐Lei Guo; Na-Nv Liu; Xu‐Guang Xi
• Keywords: G-quadruplex

Our data challenge Chen et al.'s interpretation of smFRET results, i.e. the repetitive unfolding G4 with one-base translocations. We believe that observed oscillatory curve represents alternate binding DHX36 to 3' and 5'G-tetrad G4s, rather than between canonical transformed non-canonical G4s. Noteworthily, our results reported here also call into question previously published helicase-mediated unfolding. Therefore,discriminating signal from is very important avoid misinterpreting results.