Molecular fingerprinting of biological nanoparticles with a label-free optofluidic platform

• DOI: 10.48550/arxiv.2308.06117
• Publication Date: 2023-01-01
• Authors: Alexia Stollmann; Jose Garcia‐Guirado; Jae‐Sang Hong; Hyungsoon Im; Hakho Lee; Jaime Ortega Arroyo; Romain Quidant
• Keywords: Interfacing; Digital Microfluidics; Robustness

Label-free detecting multiple analytes in a high-throughput fashion has been one of the long-sought goals biosensing applications. Yet, for all-optical approaches, interfacing state-of-the-art label-free techniques with microfluidics tools that can process small volumes sample high throughput, and surface chemistry grants analyte specificity, poses critical challenge to date. Here, we introduce an optofluidic platform brings together digital holography PDMS by using supported lipid bilayers as building block integrate both technologies. Specifically, this fingerprints heterogeneous biological nanoparticle populations via multiplexed immunoaffinity assay single particle sensitivity. Herein, first thoroughly characterise robustness performance platform, then apply it profile four distinct ovarian cell-derived extracellular vesicle over panel protein biomarkers, thus developing unique biomarker fingerprint each cell line. We foresee our approach will find many applications where routine characterisation nanoparticles is required.