Phenol biodegradation by mixed culture in batch reactor — Optimization of the mineral medium composition

• DOI: 10.5004/dwt.2011.1428
• Publication Date: 2011-01-18T09:25:36Z
• Authors: A. Hamitouche; A. Amrane; Z. Bendjama; F. Kaouah
• Keywords: 0301 basic medicine; Microbial consortium; 03 medical and health sciences; [CHIM.GENI]Chemical Sciences/Chemical engineering; Phenol; [CHIM.ANAL]Chemical Sciences/Analytical chemistry; [SDE.IE]Environmental Sciences/Environmental Engineering; Biodegradation; Batch kinetics; [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering; 6. Clean water

Abstract A new phenol-degrading microbial consortium with high biodegradation activity and tolerance to phenol was isolated from activated sludge for hazardous wastewater treatment (Boumerdès, Algeria). Biodegradation kinetics determined by monitoring biomass consumption. Mineral medium composition especially nitrogen source concentration, as well culture pH, were optimized. The temperature (30°C), the stirring velocity (200 rpm) concentration (125 mg L-1) kept constant; while initial pH varied (5–9), mineral components tested in following ranges: 0.25–2 g L-1 (NH4)2SO4,1–4 KH2PO4 NaH2PO4 0.05–0.2 MgSO4. All experiments carried out at a given bacterial 0.08 (based on optical density determination, 0.079). Irrespective of conditions, total degradation recorded times ranging 20.6 31.2 h. optimal concentrations therefore, 1,3,3 0.1 (NH4)2SO4, KH2PO4, MgSO4 respectively 8, leading specific growth rate 0.64 Ir1. Higher maximum values during this work, if compared those reported available literature, even dealing mixed culture. This result showed relevance used. Keywords: BiodegradationPhenolMicrobial consortiumBatch