Visible Surface-Assisted Laser Desorption/Ionization Mass Spectrometry of Small Macromolecules Deposited on the Graphite Plate

• DOI: 10.5012/bkcs.2002.23.2.315
• Publication Date: 2010-07-08T06:44:31Z
• Authors: Jung Hwan Kim; Kyungsoo Paek; Weekyung Kang
• Keywords: Soft laser desorption

Visible surface-assisted desorption/ionization mass spectrometry (SALDI-MS) has been investigated for several small macromolecules deposited on the graphite plate using laser radiation at 532 nm where most of are transparent. The surface functioned well as a photon absorbing material and an energy transfer mediator visible light. results show that SALDI is much softer ionization technique than UV-MALDI FAB-MS in our with synthetic macromolecules, PPG, PPGMBE cavitand molecules. For biomolecules, glycerol proton source was essential plate. As SALDI, role division cationization agent can provide generality spectrometric analysis compared MALDI dual functional matrix.