Standard operating protocol of hepatic organoid differentiation from human induced pluripotent stem cells

• DOI: 10.51335/organoid.2022.2.e5
• Publication Date: 2022-05-31T04:24:56Z
• Authors: Min Jung Kim; Jaeseo Lee; Seon Ju Mun; Myung Jin Son; Jung-Hyun Kim
• Keywords: 0301 basic medicine; 03 medical and health sciences; induced pluripotent stem cells; disease modeling; RZ409.7-999; hepatocytes; liver; Miscellaneous systems and treatments; organoids; TP248.13-248.65; Biotechnology; 3. Good health

Mature liver organoids are promising cell sources for research to understand the pathology underlying a variety of conditions affecting the liver, including end-stage chronic liver disease. Although several methods exist for the differentiation of mature hepatic organoids derived from human induced pluripotent stem cells (hiPSCs), organoid generation can fail due to various experimental culture conditions. Therefore, we established a standard operating protocol for generating mature and expandable hepatic organoids derived from hiPSCs, and we made the starting materials available to facilitate the wide use of the protocol.