Glut-1 expression in proliferative endometrium, endometrial hyperplasia, endometrial adenocarcinoma and the relationship between glut-1 expression and prognostic parameters in endometrial adenocarcinoma

• DOI: 10.5146/tjpath.2015.01352
• Publication Date: 2016-04-07T11:39:51Z
• Authors: Tuba Canpolat; Canan Ersoz; Aysun Uguz; Mehmet Ali Vardar; Aytekin Altintas
• Keywords: Adult; Glucose Transporter Type 1; Hyperplasia; GLUT-1; Endometrial hyperplasia; Middle Aged; Prognosis; Immunohistochemistry; Survival Analysis; Endometrial Neoplasms; 3. Good health; Endometrium; 03 medical and health sciences; 0302 clinical medicine; Biomarkers, Tumor; Disease Progression; Humans; Female; Endometrioid adenocarcinoma; Carcinoma, Endometrioid; Precancerous Conditions; Aged

Malignant cells show increased glucose uptake in in vitro and in vivo studies. This uptake is mediated by glucose transporter proteins. GLUT-1 is the most common transporter protein, and its expression is reported to be increase in many human cancers. The aim of this study is to determine the GLUT-1 overexpression in benign, hyperplastic, and malignant endometrial tissues, to evaluate the usefulness of GLUT-1 expression in endometrial hyperplasia, and to determine its role in the neoplastic progression to endometrioid type adenocarcinoma. We also aimed to analyze prognostic clinical parameters, predict prognosis, and survival.We examined immunohistochemical expression of GLUT-1 in 91 cases of endometrial hyperplasia, 100 cases of endometrioid type adenocarcinoma, and 10 proliferative endometrial tissues. The percentage of positive cells and staining intensity were assessed in a semi quantitative fashion and scored (1+ to 3+).GLUT-1 immunoreactivity was not present in proliferative endometrium. Twenty-nine (31.9%) of 91 endometrial hyperplasia cases showed positive immunoreactivity, of which only six were cases of hyperplasia without atypia while 23 of them were cases with atypia. We found GLUT-1 positivity of 95% in endometrioid type adenocarcinoma. GLUT-1 overexpression was not significantly correlated with any of the clinicopathological parameters except histological grade in endometrioid adenocarcinoma; the survival was not found to be correlated with GLUT-1 expression.GLUT-1 immunostaining may be useful in distinguishing hyperplasia without atypia from hyperplasia with atypia; GLUT-1 overexpression is a consistent feature of endometrioid adenocarcinoma. A correlation between GLUT -1 expression and tumor grade has been found, although other prognostic parameters and survival has no meaningful correlation.