miR-224 Regulates the Aggressiveness of Hepatoma Cells Through the IL-6/STAT3/SMAD4 Pathway

• DOI: 10.5152/tjg.2021.191056
• Publication Date: 2021-08-13T09:14:13Z
• Authors: Fangmei An; Xiongbo Wu; ; Yunan Zhang; ; Dayang Chen; ; Guoqiang Zhang; ; Yexin Lin; ; Fang Wu; ; Junli Ding; ; Min Xia; ; Qiang Zhan
• Keywords: Liver Cancer

Previous studies have shown that miR-224 regulates the progression of liver cancer. The aim this study was to investigate underlying mechanisms.The miR-224, p-STAT3 and SMAD4 expression levels were checked with tissue or/and serum samples HCC patients by qRT-PCR or IHC methods. regulatory role IL-6 in investigated Western-blot. targeted gene verified both Western-blot luciferase reporter assay. Furthermore, carcinogenesis cell experiments vitro mouse xenograft model vivo imaging vivo.It found elevated patients. higher but lower tumor tissues. Moreover, can induce p-STAT3/STAT3 cells STAT3 played bridge between miR-224. Target 3'UTR SMAD4. Finally, promoting roles miR-224in growth, proliferation, invasion migration discovered studies.It implies may potentially represent a new target for developing novel anti-HCC therapeutics.