Intestinal segment and vitamin D3 concentration affect gene expression levels of calcium and phosphorus transporters in broiler chickens

• DOI: 10.5187/jast.2022.e78
• Publication Date: 2022-10-21T07:05:25Z
• Authors: Jincheng Han; Lihua Wu; Xianliang Lv; Mengyuan Liu; Yan Zhang; Lei He; Junfang Hao; Li Xi; Hongxia Qu; Chuanxin Shi; Zhiqiang Li; Zhixiang Wang; Fei Tang; Yingying Qiao
• Keywords: Jejunum

Two experiments were conducted in this research. Experiment 1 investigated the spatial expression characteristics of calcium (Ca) and phosphorus (P) transporters duodenum, jejunum, ileum 21-day-old broilers provided with adequate nutrient feed. 2 evaluated effects dietary vitamin D3 (VD3) concentration (0, 125, 250, 500, 1,000, 2,000 IU/kg) on growth performance, bone development, gene levels intestinal Ca P 1-21-day-old negative control diet without supplemental VD3. Results experiment showed that mRNA calcium-binding protein 28-kDa (CaBP-D28k), sodium-calcium exchanger (NCX1), plasma membrane ATPase 1b (PMCA1b), IIb sodium-phosphate cotransporter (NaPi-IIb) highest broiler duodenum. By contrast, inorganic phosphate transporter (PiT-1) (PiT-2) ileum. adding 125 IU/kg VD3 increased body weight gain (BWG), feed intake (FI), weight, percentage tibia femur compared (p < 0.05). The rise from to 1,000 further BWG, FI, weights bone, ash, Ca, No difference rate leg quality was noted > Supplementation 125-2,000 abundances varying degrees. level CaBP-D28k by 536, 1,161, 28 folds ileum, respectively, after other (PMCA1b, NCX1, NaPi-IIb, PiT-1, PiT-2) 0.57-1.74 1,000-2,000 These data suggest are mainly expressed duodenum broilers. Moreover, addition stimulates two mineral transcription intestines.