EVALUATI ON OF THE DISK DIFFUSION METHOD FOR TESTING FLUCONAZOLE SUSCEPTIBILITY OF Cryptococcus laurentii

• DOI: 10.5216/rpt.v42i1.23592
• Publication Date: 2013-04-11T14:50:57Z
• Authors: Reginaldo S. Pedroso; Joseane C. Ferreira; Karen R. C. Costa; Regina C. Candido
• Keywords: Broth microdilution; Agar diffusion test

Cryptococcus laurentii is a rare human pathogen ubiquitous in nature. This study aims to evaluate the disk diffusion method for testing fluconazole susceptibility of C. laurentii, and moreover, assess minimal fungicidal concentration (MFC) by broth microdilution method. Eleven isolates environmental complex were employed determine methods according CLSI (Clinical Laboratory Standards Institute) guidelines MFC technique. Thedisk indicated four susceptible, three susceptible-dose dependent resistant while 10 defined as susceptible one dependent. The agreement between was 36.4%. One isolate showed 8 ?g/mL two 64 ?g/mL. Although only small number studied, results suggested that not adequate vitro isolates, fluconazole, it fungistatic antifungal, may present activity some isolates.