Production of enterotoxins of Staphylococcus spp. isolated from samples of sheep milk

• DOI: 10.5219/361
• Publication Date: 2014-05-06T20:30:07Z
• Authors: František Zigo; Milan Vasiľ; Juraj Elečko; Martin Lapin; Zuzana Farkašova
• Keywords: Udder; Coagulase; Subclinical infection

In our study was followed occurrence of mastitis in herd 430 sheep breed zoslachtena valaska with hand milking technology examined two times during one lactation season. Individual examination consisted from clinical udder and microbiological milk samples. By PCR determined presence genes coding production enterotoxins, by ELISA methods individual types enterotoxins. From forms were frequently detected subacute (6.7%), subclinical (5.7%) acute (2.9%). The coagulase-negative staphylococci (CNS) identified 102 (65.4%) all 156 positive isolates. CNS S. aureus caused (5.1%), (3.9%) (2.4%) mastitis. most isolated epidermidis, chromogenes xylosus ewes chronical predominantly aureus, uberis epidermidis. staphylococcal enterotoxins (SE) - SEA, SEB, SEC, SED the sec (3), sea (2), seb (2) sed schleiferi chromogenes, respectively. results suggested on high (12.4%) forms. Confirmed their present a risk for human health decreased quality products sheep´s milk.