Evaluation of Diagnostics Colistin MIC-Strip Test for Colistin Susceptibility Testing

• DOI: 10.5222/ankem.2021.009
• Publication Date: 2021-05-03T12:07:44Z
• Authors: Gülşen Altınkanat...; Elvan Sayın; Ufuk Hasdemir; Güner Söyletir
• Keywords: Colistin; Broth microdilution; Acinetobacter baumannii

Due to the limited number of antimicrobials be used in treatment infections caused by Gram-negative microorganisms with multi-drug resistance recently, old antibiotics such as colistin have started preferred frequently. However, some problems are encountered antibiotic susceptibility tests (disk diffusion, gradient test, automated systems) which frequently routine laboratory due cationic nature colistin. For this reason, only broth microdilution test is recommended European Committee on Antimicrobial Susceptibility Testing (EUCAST) and Clinical Laboratory Standards Institute (CLSI) for detection susceptibility. Since time consuming inconvenient, that can provide fast reliable result needed laboratories. In study, it was aimed evaluate performance commercially produced Diagnostics MIC-COL (Diagnostics I.n.c, Galanta, Slovakia) Klebsiella pneumoniae Acinetobacter baumannii strains. The strains K.pneumoniae (n = 22) A.baumannii 28) isolated from various clinical specimens between 2016 2019 our were included study. Colistin minimum inhibitory concentrations (MIC) studied both reference method test. essential agreement, categorical major error, very error rates calculated comparing obtained results. agreement MIC-Strip determined 84 %, 98 rate 3.8 while no detected. İt important guide antimicrobial rapid commercial study easy use not time-consuming. Also, its strip from, each isolate separately. Because being above expected values, will useful re-evaluate these further studies conducted a larger different levels.