Investigation of Phospholipase Activity in Candida albicans Strains Isolated From Blood and Oral Cavity Specimens

• DOI: 10.5222/tmcd.2021.84429
• Publication Date: 2021-03-22T09:01:38Z
• Authors: Buğse Tunç; Ebru Demiray Gürbüz; Mine Doluca Dereli
• Keywords: Phospholipase A

Objective: The aim of the present study was to investigate and compare phospholipase B1, B2, C1 D1 activities in C.albicans strains isolated from blood cultures oral cavity specimens. Method: Phospholipase activity examined by plate method reverse transcriptase polymerase chain reaction (RT-PZT). Results: Twenty-six (86.7%) 24 (80.0%) cavities revealed method. No statistically significant difference (χ2 =0.48; p=0.49) observed between groups. However statistical determined mean Pz values (0.6138±0.9823; 0.6988±0.9910) (p=0.007). PLB1 expression detected all (100%), PLB2 29 (96.7%) 30 (100.0%), PLC1 27 (90%) 22 (73.3%), PLD1 (90.0%) 21 (70.0%) strains, respectively. While no groups (t=-0.307; p=0.760), expressions were found be significantly higher (p=0.043) isolates (p<0.001), (p=0.732). PLB1, PLB2, 100%, 81.7%, 71.6% 76.7% agreement with 83.3% when genes considered. correlation (p=0.602; p=0.555; p=0.241; p=0,096, respectively). Conclusion: high rates our study, support important roles these enzymes virulence. Our results may indicate that encoded play more for invasive infections, respectively; however large scale studies are needed on this issue.