Developing a highly validated and sensitive HPLC method for simultaneous estimation of cefotaxime and paracetamol in pure and pharmaceutical preparations

• DOI: 10.5267/j.ccl.2023.10.002
• Publication Date: 2023-12-25T05:22:42Z
• Authors: Mahmoud M. Sebaiy; Sobhy M. El-Adl; Samar S. Elbaramawi; Shaban A. A. Abde...; Alaa Nafie
• Keywords: Distilled water; Vial

An isocratic HPLC technique was exploited and validated for the quick simultaneous separation measurement of cefotaxime paracetamol in vials dosage forms, with a total analysis time 3 minutes. The process carried out on Thermo Scientific® Venusil XBPC18 (L) (5µm, 4.6x250 mm) using mobile phase ACN: distilled water (70:30, v/v) at ambient temperature. flow rate used experiment 1 mL/min, highest level absorption determined by high-performance liquid chromatography photodiode array detection (HPLC-PDA) employing PDA detector set wavelength 255 nm. established retention times were 1.79 2.97 minutes, respectively, suggesting reduced duration. observed limits ceftaxime 4.2×10-5 1.2×10-5 µg/mL, indicating significant sensitivity approach. approach subsequently verified accordance requirements Food Drug Administration (FDA) quantification medicines vial form.