Gingival Medicinal Signaling Cells Conditioned Medium effect on the Osteoclast and Osteoblast number in Lipopolysaccharide-induced Calvaria Bone Resorption in Wistar Rats’ (Rattus novergicus)

• DOI: 10.52711/0974-360x.2021.00911
• Publication Date: 2021-11-20T11:07:01Z
• Authors: Nastiti Faradilla...; Alexander P. Nugraha; Igo S. Ihsan; Yoni A. Agung; Fedik A. Rantam; Diah S. Ernawati; Rini D. Ridwan; Ida B. Narmada; Arif N. M. Ansori; Suhaila Hayaza; Tengku N.E.B.T.A....
• Keywords: Calvaria

The gingival medicinal signaling cells conditioned medium (GMSCs-CM) is a biocompatible material which possessed beneficial cytokine, anti-microbial peptide, growth factor that can be collected after culture. GMSCs- CM may inhibit bone resorption in order to improve the patient’s quality of life. In this study, potential effect GMSCs-CM on number osteoclasts and osteoblasts Lipopolysaccharide (LPS)-induced calvaria wistar rats (Rattus novergicus) has been analyzed. Twenty-eight male healthy (R. at age 1-2 months old with 250-300 grams body weight were divided into 4 groups, namely PBS group: 100μg day 1-7; LPS GMSCs + GMSCS-CM 1 1-7, M-GMSCs 1-7. Escherichia Coli was used induce subcutaneous injection. passage 4-5 then injected subcutaneously calvaria. All samples examined the. 8th through cervical dislocation. observed under 400x magnification. One Way ANOVA Tukey HSD conducted analyze differences between groups (p<0.01). decreased significantly group compared increased reduce amount osteoclast increases production osteoblast LPS-induced novergicus).