Anti-Aging Effects of Retinol and Alpha Hydroxy Acid on Elastin Fibers of Artificially Photo-Aged Human Dermal Fibroblast Cell Lines

• DOI: 10.5281/zenodo.1100102
• Publication Date: 2015-03-05
• Authors: Mohammed Jarrar; Shalini Behl; Nusrat Shaheen; Aleena Fatima; R. Nasab
• Keywords: Dermal fibroblast; Alpha (finance)

{"references": ["S. Ronken, \"The effect of ascorbic acid and retinoic acid on elastin and\ncollagen gene expression in monolayers\" Identity number: 0527700\nBMTE. Accessed from http://www.mate.tue.nl/mate/pdfs/8073.pdf,\n2007.", "A. Hayashi, T. Suzuki, and S. Tajima, \"Modulations of elastin\nexpression and cell proliferation by retinoids in cultured vascular smooth\nmuscle cells,\" J. Biochem., vol. 117, pp. 132\u2013136G. Jan 1995.", "J. William, B. Timothy, \"Diseases of the Skin: clinical Dermatology\",\nSaunders Elsevier. ISBN 0-7216-2921-0, 2006.", "L. D. Muiznieks, A. S. Weiss and F.W. Keeley, \"Structural disorder and\ndynamics of elastin\", Biochem Cell Biol 88 (2): 239\u201350, 2010.", "J. Uitto, \"The role of elastin and collagen in cutaneous aging: Intrinsic\naging versus photoexposure\", J Drugs Dermatol. 2008 Feb; 7 (2 Suppl):\ns12-6, 2008.", "E. F Bernstein, Y. Q Chen, K. Tamai, K. Shepley, K. S. Resnik, H.\nZhang, R. Tuan, A. Mauviel, J. Uitto, \"Enhanced elastin and fibrillin\ngene expression in chronically photodamaged skin\", J Invest Dermatol\n103:182-186, 1994.", "G. A. Di Lullo, S. M. Sweeney, J. K\u00f6rkk\u00f6, L. Ala-Kokko, J. D. San\nAntonio, \"Mapping the Ligand-binding Sites and Disease-associated\nMutations on the Most Abundant Protein in the Human, Type I\nCollagen\", J. Biol. Chem. 277 (6): 4223\u20134231, 2002.", "M. Ramos, E. Silva, D. M. Hexsel, M. S. Rutowitsch, \"Hydroxy acids\nand retinoids in cosmetics, \"ClinDermatol; 19:460\u20136, 2001.", "E. J. Van Scott, R. J. Yu, \"Alpha hydroxy acids: Procedures for use in\nclinical practice\", 43:222\u20138, 1989.\n[10] C. M. Ditre, T.D Griffin, G. F. Murphy, E. J. Van Scott, \"Improvement\nof photodamaged skin with alpha-hydroxy acid (AHA): A clinical,\nhistological, and ultra-structural study\", Dermatology Congress. Vienna,\nAustria. May 18\u201321, 1993:175, 2000.\n[11] X. Wang. \"A theory for the mechanism of action of the alpha-hydroxy\nacids applied to the skin\", Medical Hypotheses; 53:120 \u20132, 1999.\n[12] M. N. O'Donoghue. \"Update on acne therapy\". Dermatol Nurs; 11:205\u2013\n8, 1999."]}<br/>Skin aging is a slow multifactorial process influenced by both internal as well as external factors. Ultra-violet radiations (UV), diet, smoking and personal habits are the most common environmental factors that affect skin aging. Fat contents and fibrous proteins as collagen and elastin are core internal structural components. The direct influence of UV on elastin integrity and health is central on aging of skin especially by time. The deposition of abnormal elastic material is a major marker in a photo-aged skin. Searching for compounds that may protect against cutaneous photodamage is exceedingly valued. Retinoids and alpha hydroxy acids have been endorsed by some researchers as possible candidates for protecting and or repairing the effect of UV damaged skin. For consolidating a better system of anti- and protective effects of such anti-aging agents, we evaluated the combinatory effects of various dosages of lactic acid and retinol on the dermal fibroblast’s elastin levels exposed to UV. The UV exposed cells showed significant reduction in the elastin levels. A combination of drugs with a higher concentration of lactic acid (30 -35 mM) and a lower concentration of retinol (10-15mg/mL) showed to work better in maintaining elastin concentration in UV exposed cells. We assume this preservation could be the result of increased tropo-elastin gene expression stimulated by retinol whereas lactic acid probably repaired the UV irradiated damage by enhancing the amount and integrity of the elastin fibers.<br/>