Effects of Finite Deformation on Extracellular Matrix Production and Mechanical Properties

• DOI: 10.5339/qproc.2012.heartvalve.4.13
• Publication Date: 2012-07-05T15:42:05Z
• Authors: John A. Stella; Nicholas J. Amoroso; Antonio D’Amore; John E. Mayer, Jr.; William R. Wagner; Michael S. Sacks
• Keywords: Matrix (chemical analysis); Strain (injury); Mechanical load

Abstract With our ability to incorporate viable cells distributed throughout the scaffold, we are provided a unique, controllable platform develop generalized finite deformation framework than can be used gain an understanding of how evolving extracellular matrix phase contributes construct gross mechanical behavior. The biosynthetic response microintegrated VSMC’s was investigated low (15%), intermediate (30%), and high (50%) strain groups. These magnitudes were chosen as they correspond wide range NAR deformations physiologically relevant. A constant, quasi-static rate applied sufficient obtain 1 Hz cycle duration. Culture durations 7, 14, 21 day time points for each level quantify ECM synthesis capacity VSMC in electrospun PEUU. static group also preformed at period. Results indicate that behavior is function global with peaked soluble collagen observed specimens exposed 30% strain. Our primary goal elucidate characteristics de-novo formed ECM. We determined contribution by assuming total simply summation individual phases any potential interactions might arise between them. thus quantified mass fraction utilized enzymatic technique remove from constructs, then retested them degraded scaffold only responses. indicated newly exhibit highly anisotropic biaxial response, over 100 fold stiffer similar using stiff scaffolds previously studied lab. Moreover, had predicted properties glutaraldehyde treated pericardium, common heart valve biomaterial. Interestingly, peak activity correlates well vitro principle levels PEUU tri-leaflet valves native ovine right side pressure. This suggests physiologic hemodynamic conditions optimal development robust accretion. To knowledge, this first reported study consider effects large corresponding outcomes terms integrity. Furthermore, results reveal interesting insights into functional role accretion process engineered tissues.