Tanshinone IIA Ameliorates H2O2-induced Antioxidant Effects in Huh7 Cells

• DOI: 10.54097/s046w005
• Publication Date: 2025-05-21T00:06:05Z
• Authors: Dongrui Huang; Mingze Sun; Tiancheng Xu; Pan Peng; Wenjing Liao; Fang Wu; Meng Fang; Yuwei Liu

Objective This study aims to elucidate the antioxidative mechanisms of tanshinone IIA (Tan IIA) in ameliorating hydrogen peroxide (H2O2)-induced oxidative stress Huh7 cellular model. Methods The association between Tan and HCC was analyzed using network pharmacology techniques. Changes activity on H2O2-induced cells related antioxidant indices were determined CCK-8. underwent analysis Western blot immunofluorescence assessments delineate concentrations PI3K/Akt Nrf2/HO-1 proteins their corresponding interactions. Results Network pharmacological demonstrated multi-targeting properties for treatment HCC. state significantly alleviated with intervention IIA, levels superoxide dismutase (SOD) elevated, malondialdehyde (MDA) level decreased, glutathione peroxidase (GSH-Px) elevated. Meanwhile, experiments revealed that promoted Nrf2 HO-1 proteins, ameliorated phenomenon, inhibitory effect phosphorylation PI3K Akt. Conclusions Our investigation indicates modulates through suppression stimulation signaling cascade.