Characterization of Thermoplasma Species Cultured from Sampling on Tangkuban Perahu, Indonesia

• DOI: 10.5454/mi.8.1.3
• Publication Date: 2015-03-14T16:09:25Z
• Authors: AMARILA MALIK; IMAN SANTOSO; ANDI YEHUDA; SERUNI K.U. FREIS...; SEPTELIA I INAWAT...; HARALD HUBER; ZESSINDA LUTHFA; ROSARI SALEH; HANS-JOACHIM FREI...
• Keywords: Thermoplasma acidophilum

Archaea is an organisme with unique feature because of its ability to inhabit extremophyle conditions. Our expeditions Tangkuban Perahu, West Java aimed obtain archaealstrains from the solfatara fields located in Domas crater. From samples, we intended culture Thermoplasma species growing around 55 °C below pH 2, which until now have not yet been fully characterized. We collected five samples mud holes temperatures 52 57 and 2. In serial cultures up 8 transfers Freundt's medium grew tetraetherlipid synthesizing as documented by phase contrast microscope. Total membrane lipid extracts were analysed thin layer chromatography; pattern matched total acidophilum DSM 1728 membranes. For confirmation, 16S rDNA identification performing PCR sequencing carried-out. Analysis using BLAST showed T. identities highest similarity 99%, followed volcanium, also with99% (ANKF776908 ANKF776909). This first report culturing cell-wall-less thermoacidophilicarchaea,in particular Indonesian laboratories.