Isolation and characterization of strictly anaerobic cellulolytic rumen bacterial species from Sahiwal cattle

• DOI: 10.5455/javar.2024.k740
• Publication Date: 2024-03-13T21:18:11Z
• Authors: Muhammad Alam; Md Hossain; M Sohidullah; Md Khan; Khan Islam
• Keywords: Isolation

Objective: To isolate, and characterize cellulolytic rumen bacteria from the of Sahiwal cattle utilizing bacterial inoculum to increase nutritional value rice bran used as broiler feed. Materials Methods: The ruminal liquid was kept at an optimal pH 6.9, a redox potential less than -300mV while being incubated anaerobically 39℃ in medium containing Rumen Fluid Glucose Cellobiose Agar. By using Hungate technique, isolates were detected on basis their morphological, physiological, biochemical, molecular testing. Results: findings revealed that isolated Ruminococcus albus, flavifaciens obligate anaerobic, generally Gram-positive, non-motile cocci or rod, single pair, occasionally short chain, producing yellow pigment when grown cellulose, presence clear zone around colonies. Both fermented sugars such cellobiose, glucose, lactose well decomposed xylan. results also showed recognized spp., bacterium, catalase negative, indole gelatin liquefaction positive. Conclusion: Isolation characterization spp. may be helpful for Bangladesh reducing cost poultry feed, circumventing restrictions use. We can develop more efficient long-lasting plans enhance performance, feed efficiency through understanding how various function this process.