Inhibition of Cytochrome P450 by Propolis in Human Liver Microsomes

• DOI: 10.5487/tr.2016.32.3.207
• Publication Date: 2016-07-13T02:04:13Z
• Authors: Chang Seon Ryu; Soo Jin Oh; Jung Min Oh; Ji-Yoon Lee; Sang Yoon Lee; Jung-woo Chae; Kwang-il Kwon; Sang Kyum Kim
• Keywords: Propolis

Although propolis is one of the most popular functional foods for human health, there have been no comprehensive studies herb-drug interactions through cytochrome P450 (CYP) inhibition. The purpose this study was to determine inhibitory effects on activities CYP1A2, 2A6, 2B6, 2C9, 2C19, 2D6, 2E1 and 3A4 using pooled liver microsomes (HLMs). Propolis inhibited CYP2E1 CYP2C19 with an IC50 value 6.9, 16.8, 43.1 μg/mL, respectively, whereas CYP2A6, were unaffected. Based half-maximal concentration shifts between incubated without nicotinamide adenine dinucleotide phosphate, propolis-induced CYP2C19, inhibition metabolism-independent. To evaluate interaction potential therapeutic drugs, metabolism duloxetine, a serotonin-norepinephrine reuptake inhibitor, determined in HLMs. CYP1A2 CYP2D6 are involved hydroxylation duloxetine 4-hydroxy major metabolite, which decreased following addition These results raise possibility drugs metabolized by CYP1A2.