Investigation of toxin genes and potential to control early shrimp mortality disease of chitosan on some Vibrio parahaemolyticus strains isolated from Mekong Delta

• DOI: 10.54939/1859-1043.j.mst.fee.2024.261-267
• Publication Date: 2024-12-17T04:46:11Z
• Authors: Dang Phuong Nam; Le Minh Tri; Le To Lan; Nguyen Thi Huyen; Doan Van Cuong; Nguyen Thi Nhung
• Keywords: 0301 basic medicine; 03 medical and health sciences

Acute Hepatopancreatic Necrosis Disease (AHPND) is a disease that induces mass early mortality in shrimp caused by Vibrio parahaemolyticus. This bacterium capable of secreting biofilm - the main element host invasion mechanism and also protects V. parahaemolyticus from anti-bacterial agents, especially antibiotics. In this study, 03 strains isolated infected samples were investigated for toxin genes. They had toxR gene lacked tdh, trh pPVA3-1 virulence plasmid Chitosan was used to inhibit destroy their biosynthesis order prevent AHPND. The minimum chitosan concentration inhibiting 2 g/L. At 3 g/L, destroying 87.78 88.74% formed film. Biofilm analysis before after treatment with showed EPS rate reduced 68-72.73% compared treatment. results demonstrated potential prevention