Study of the Association between Helicobacter Pylori Infection and Primary open angle Glaucoma in China

• DOI: 10.55124/ijim.v1i1.30
• Publication Date: 2022-06-13T07:02:00Z
• Authors: Jianwei Zhou
• Keywords: Association (psychology)

Objective: To assess the relationship between Helicobacter pylori (Hp) infection and primary open-angle glaucoma (POAG); meantime, to explore possible mechanism of POAG induced by Hp. Methods: 30 consecutive patients, angle-closure (PACG) cataract patients were recruited divided into three groups according different diseases. The sera aqueous humor samples collected used detect Hp-specific IgG antibody (Hp-Ab) with dot immunogold filtration assay (DIGFA). 14C-urea breath test (14C-UBT) was carried out Hp all participants. Results: Hp-Ab positive rate respectively 76.7% (23/30) 66.7% in for group, which significantly higher than corresponding data other two (all P<0.05). In 14C-UBT, 63.3% group it close that serological result detected DIGFA (P>0.05). There little numbers ANA ENA no meaning make statistically analysis. Conclusions: is association POAG, autoimmune suggested as one key mechanisms our opinions.
 Introduction
 Glaucoma commonest causes blindness world. Generally, (POAG) (PACG).1 As a leading blindness, study more attention.2,3To understand, chronic optic neuropathy characterized atrophy increased cupping disk. date, many aspects its pathogenesis remain unknown but some significant risk factors are advanced age, African origin, familial history elevated intraocular pressure.4,5
 Gram-negative microaerophilic bacterium plays an important role development various upper gastrointestinal With studies, researchers reported also associated extragastric diseases, such ischemic heart disease,6 iron-deficient anemia,7 diabetes mellitus,8 so on. 2001, Kountouras et al9 established prevalence Greek population, causal link glaucoma. Subsequently, this finding evidenced scholars their own studies.10 But significance remains uncertain because conflicting findings studies.11-13 Aiming discrepancy, further studies necessary.14
 study, we just do antibodies ocular including PACG cataract, attempt determine analyze Hp.
 Abbreviations
 ANA, antinuclear antibody; ENA, Extractable nuclear antigen; DIGFA, assay; Hp, pylori; Hp-Ab, antibodies; PACG, glaucoma; 14C-UBT: test.
 
 Subjectsand methods
 Subjects
 enrolled average age 68±7.3 y (ranged from 47 78 y). ratio male female 11: 19. Meantime, recruited, who matched sex patients. According participants groups, respectively. All them excluded tumor, immunodeficiency, infectious diseases clinic, had antibiotics medicines related immunopotentiator or immunosuppressive agents six months before experiment. Written informed consents obtained approved local ethics committee.
 detection samples
 2 ml venous blood each serum after centrifugation (DIGFA) manufacturer’s instruction reagent kit (MP Biomedicals Asia-Pacific Pte. Ltd., Singapore).
 About 50 μl sample aspirated at beginning surgery assayed same process samples.
 Detection test
 Referring Tang’s report,1514C-urea instrument-YH04 (Yanghe Medical Equipment Co. China).
 Sera auto-antibodies detection
 Serum (ANA) indirect immunofluorescence commercialized kit. antigen (ENA) line immunoassay. reagents bought Jiangsu HOB Biotech Group, China.
 Statistic analysis
 Using T-test Chi-square test, analyses performed SPSS 13.0 software. P value less 0.05 considered significant.
 Results
 3.1 Of samples, there 23 cases exhibited Hp-Ab-positive those (43.3% 36.6% respectively). 18 66.7%. Compared difference (Table 1). mean similar existed (P = 0.287).
 Table 1. qualitative results glaucoma
 14C-UBTAH: humor; a: vs group; b: c: group.
 19 Hp-Ab-positive, 63.3%. not 2).
 2. Comparison 14C-UBT diagnosis group
 detection* represents comparison methods.
 4, 1 ANA-positive SSA, SSB Ro-52, 2, Only Ro-52 showedpositive while 3).
 3. Discussion
 Greece, very active research led J. published several original contributions well reviews concerning connection POAG.14,16 counties, papers containing arguments issued, India,17 Turkey,18 Korea19 China, Hong al20 through 13C-UBT, found correlation them. Since then, relative article issued Chinese could be PubMed well-known scientific database. referring researchers’ reports, designed experiments. results, high groups. This previous reports2,21 verified relation POAG.
 present 14C-UBT. Encouragingly, 63.3%, DIGFA. indicated existence POAG. However, Bagnis al22 thought based on assessment might misleading, since sensitive markers infection; 13C-UBT clarify actual among infected fact, still deficiencies 13C- 14C -UBT, suitable infection, extent, false-negatives test.23 probably reason what study. cresyl fast violet staining histology preparations tissue trabeculum iris introduced Zavos al,24 although provide direct strong evidence pathophysiology difficult harvest limited application. Therefore, opinions, infectionand Except detecting majority shown, consistent again showed another Deshpande al17 controls concentration they did find any correlations concentrations patient disagreement damage degree blood-brain barrier (BBB), reach under condition BBB disruption.25 supported hypothesis onset circulation get blood-aqueous barrier, condensed finally aggravated glaucomatous damage.2
 occurrence most probable should ignored: initiated response common genetic components shared human nerve tissue; cell destruction mediated apoptosis caused glaucoma.26 Just theory, control participants, hoped evidences autoimmune. result, every rather low seronegative can’t deny POAG; specific eyes, iris, future Conclusion
 only using And further, experimental data, onset, taken routinized index applied prevention therapy clinic. can sufficiently investigate relates infection. Is true causative factor POAG?18 What initial if pathogen takes part disease? Such questions will topics medical worldwide future.
 Funding
 work Research Fund Lin He’s Academician Workstation New Medicine Clinical Translation Jining University(JYHL2018FMS08), Project support fund teachers University (JYFC2018FKJ023).
 Conflicts interest
 conflict interest authors.
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