Evaluation and Optimization of Effective-dose of Alloxan for Inducing Type-2 Diabetes Mellitus in Long Evans Rat

• DOI: 10.5530/ijper.51.4s.96
• Publication Date: 2018-01-29T04:43:23Z
• Authors: Shaikh Shahinur R...; Nilufar Yasmin; Abu Taher Mohamma...; Akteruz zaman; Mohammad Hafizur ...; Shakh Mohammad Ab...
• Keywords: Alloxan; Intraperitoneal injection; Reversion; Evans Blue

Abstract: Background: The optimal effective and safe dose of alloxan for inducing stable diabetes in rats has been long arguable. Lower doses can result auto-reversion from diabetogenic state to normal state. On the other hand, higher cause toxicity loss experimental animals since it completely destroys insulin-producing pancreatic β cells. Therefore, determination as well induce Long Evan is crucial investigating on diabetes. Objective: To determine a mellitus Evans Rats facilitate availability diabetic studying Methods: Healthy adult (80-156 g) were divided into eight groups each group contains six rats. One treated nondiabetic control (C) while seven (Group-2 Group-8) used groups. Different (80, 100, 120, 130, 140, 150 160 mg/kg body weight) injected through intraperitoneal routes overnight fasting group-2 group-8 respectively. Blood glucose levels monitored before after administering alloaxan (for 7 consecutive weeks) by using blood meter test strips. Results: In case non-diabetic rats, found between 7.75 10.8 mmol/L. slow gradual increment measured among (group-2, group-3 group-4) with low 100 120 BW) until 5 weeks. However, increased reverted back its following two Group-5 Group-6 130 140 respectively stably during periods. While group-7 (treated expired period might be due severe toxicity. more preferable because level much significant than group-5. Conclusion: optimum was BW induction Key words: Alloxan, Diabetes mellitus, Rat, induced diabetes, Toxicity.