Toxicity and Genotoxicity in HeLa and E. coli Cells Caused by a Helium Plasma Needle

• DOI: 10.5539/apr.v5n5p21
• Publication Date: 2013-08-01T04:39:50Z
• Authors: E. Garcia-Alcantara; R. Lopez-Callejas; J. Serment-Guerrero; R. Pena-Eguiluz; A. E. Munoz-Castro; B. G. Rodriguez-M...; A. Mercado-Cabrera; R. Valencia-Alvarado; A. De-la-Piedad-B...; J. M. E. Contrera...; A. Barbabosa-Pliego
• Keywords: 0103 physical sciences; 01 natural sciences

The aim of this study is to determine the toxic and genotoxic damages produced by a helium plasma needle upon HeLa and E. coli (OG100 and PQ30) cell cultures. For HeLa cells survival (MTT) and microelectrophoresis comet) assays were performed; meanwhile in E. coli, viable count and genotoxicity by the chromotest were evaluated. The outcomes indicate that the plasma exposures on HeLa cells undergo more toxicity and genotoxicity as treatment time increases. With respect to E. coli, plasma exposure generated toxicity, but no genotoxicity could be detected with this system. In the strain OG100, defective in a protection mechanism to oxidizing agents, there was a reduction in the survival of one order of magnitude compared to the wild type strain PQ30. It suggests that such reduction is due to the plasma by means of the reactive oxygen and nitrogen species (RONS) generated during atmospheric air interaction.