Investigation of Carbapenemases in Carbapenem-Resistant Escherichia coli and Klebsiella pneumoniae Strains Isolated in 2014 in Turkey

• DOI: 10.5578/mb.10695
• Publication Date: 2016-06-11T08:38:24Z
• Authors: Aslı ÇAKAR; Yakut AKYÖN; Deniz GÜR; Onur KARATUNA; Dilara ÖĞÜNÇ; Betil ÖZHAK BAYSAN; Nilay ÇÖPLÜ; Mustafa ÇAĞATAY; Abdullah KILIÇ; Mehmet BAYSALLAR; Zahir BAKICI; Cem ÇELİK; Zeynep GÜLAY; Şöhret AYDEMİR; Alper TÜNGER; Hüseyin KILIÇ; Barış Derya ERÇAL; Zulal AŞÇI TORAMAN; Yasemin ZER; Ayşe BÜYÜKTAŞ; Selma AY; Zerrin AKTAŞ; Çiğdem KAYACAN; Gülçin BAYRAMOĞLU; Faruk AYDIN; Devrim DÜNDAR; Ufuk HASDEMİR; Ramazan AYAŞ; Keramettin YANIK; Murat GÜNAYDIN; Hüseyin GÜDÜCÜOĞLU; Mehmet PARLAK
• Keywords: Ertapenem; Carbapenem; Broth microdilution; Carbapenem-resistant enterobacteriaceae

Carbapenems are the choice of treatment in infections caused by multidrug resistant Enterobacteriaceae. In recent years carbapenem-resistant Enterobacteriaceae isolates due to carbapenemases have been increasingly reported worldwide. Multicenter studies on scarce Turkey. The aim this study was determine distribution from different parts Turkey as a part European Survey Carbapenemase Producing (EuSCAPE) project. Beginning November 2013, at least one agents, namely imipenem, meropenem, and ertapenem were sent coordinating center. Minimum inhibitory concentrations for these carbapenems determined microdilution tests following EUCAST guidelines. Production carbapenemase confirmed combination disk synergy tests. Types investigated using specific primers VIM, IMP; NDM, KPC OXA-48 genes multiplex polymerase chain reaction. six month period, 155 suspected carbapenemase-positive center which 21 (13.5%) E.coli 134 (86.5%) K.pneumoniae. Nineteen (90.5%) strains among 124 (92.5%) K.pneumoniae shown harbour gene molecular tests, with total 92.3% (143/155). Carbapenemases single enzyme 136 (OXA-48: 84.6%; NDM: 6.3%; VIM: 2.8%; IMP: 1.4%) seven (OXA-48 + 2.1%; VIM 0.7%). not detected any isolates. According test results, resistance meropenem 59.5%, 52.9% 100%, respectively. 100% compatible results. As most producing susceptible but all ertapenem, seems be sensitive agent screening areas where is prevalent phenotypic can useful centers available.