Evaluation of EUCAST Direct Rapid Antimicrobial Susceptibility Test Method in Blood Culture Bottles with Positive Signal

• DOI: 10.5578/mb.20219713
• Publication Date: 2021-10-19T07:44:31Z
• Authors: Gizem Erdoğan; Ayşe Esra Karakoç; Mihriban Yücel; Serap Yağcı
• Keywords: Gram staining; Blood Culture; Etest; Gram-Positive Bacteria; Agar diffusion test; Gram-Negative Bacteria

Early reporting of the antibiotic susceptibility testing (AST) results is essential for survival sepsis patients. In 2019, European Committee on Antimicrobial Susceptibility Testing (EUCAST) published a proposal to detect antimicrobial from positive blood culture bottles with rapid test (RAST) method in maximum eight hours. this study, it was aimed evaluate EUCAST RAST that resulted signal BacT/ALERT (bioMérieux, France) system and showed gram-negative bacteria single morphology Gram stain. The study conducted prospectively between April 2019 November 2019. Ninety we detected gram negative by stain were tested according method, isolates obtained studied disk diffusion Vitek 2 Compact (bioMerieux, automated system. compared these methods. turn around time recorded. Categorical agreement conventional methods very major error rates determined. Of 14 not yet covered HADT 12 determined be other Enterobacterales members two as non-fermentatives. Two same morphological characteristics bottle profile, but different identification results. These sixteen excluded study. 74 breakpoint tables, which 31 Klebsiella pneumoniae, 35 Escherichia coli, four Acinetobacter baumannii Pseudomonas aeruginosa. According evaluation periods RAST; profile reported nine (12%) E.coli at hours, (11%) six 18 (24%) hours; three (4%) K.pneumoniae 16 (21%) (16%) P.aeruginosa one (1%) A.baumannii (2%) hours categorical aggrement 91.8% 96.8% disc method. Very errors piperacillin-tazobactam (17.7%), ceftazidime (11.6%) meropenem (5.6%); when cefotaxime (5.7%) (6.7%). Our have shown can practicaly performed routine laboratories report early low cost. Because necessary confirm standard