Serotype Distrubution and Antibiotic Susceptibilities of Streptococcus pneumoniae Causing Acute Exacerbations and Pneumonia in Children with Chronic Respiratory Diseases

• DOI: 10.5578/mb.5655
• Publication Date: 2013-11-20T14:46:15Z
• Authors: Gülşen ALTINKANAT...; Ahmet SOYSAL; Canan KUZDAN; Bülent KARADAĞ; Ufuk HASDEMİR; Mustafa BAKIR; Güner SÖYLETİR
• Keywords: 0301 basic medicine; 0303 health sciences; Adolescent; Genotype; Respiratory Tract Diseases; Infant; Pneumonia, Pneumococcal; Pneumococcal Infections; Anti-Bacterial Agents; 3. Good health; Pneumococcal Vaccines; 03 medical and health sciences; Phenotype; Streptococcus pneumoniae; Disk Diffusion Antimicrobial Tests; Child, Preschool; Acute Disease; Chronic Disease; Drug Resistance, Bacterial; Humans; Macrolides; Serotyping; Child; Multiplex Polymerase Chain Reaction

This study aimed to investigate serotype distribution and antimicrobial resistance of Streptococcus pneumoniae isolates obtained from children with chronic respiratory diseases admitted hospital a diagnosis acute exacerbations between 2008-2010 at Marmara University Hospital, Istanbul, Turkey. Sixty one S.pneumoniae strains isolated the samples patients were studied for erythromycin, clindamycin, tetracyline, trimethoprim-sulphametoxazole (TMP-SMX), vancomycin, levofloxacin susceptibilities by disk diffusion method; MIC values penicillin ceftriaxone determined E-test (AB Biodisk, Sweden). Results evaluated according CLSI standards. The erythromycin-clindamycin double disc method was applied detection macrolide phenotypes. presence genes, ermB, mef(A)/(E), ermTR PCR using specific primers each gene. serotypes multiplex 40 different serotypes. According criteria, in found be 8.2% (5/61) intermediate rate 54% (33/61) oral penicillin. Penicillin only 1.6% (1/61) parenteral Resistance rates TMP-SMX detected as 55.8%, 46%, 47.5% 67.2%; respectively. No vancomycin levofloxacin. Constitutive macrolide-lincosamide-streptogramin B (cMLSB) phenotype M observed 82.4% (n= 28) 17.6% 6) resistant isolates, Inducible (iMLSB) not detected. macrolid genotypes, positive 50% 14.7%; Both ermB mef(A)/(E) genes 35.3% isolates. None most common 19F, 23F 6, furthermore (34%, 15.7% 18.4%, respectively) (38.2%, 20.6% 14.7%, those relatively high. Serotype covarage 7-, 10-, 13-valent conjugated pneumococcal vaccines 23-valent vaccine 65%, 67%, 69%, 78.6%, In our country, use these coverage has been effective exposed intensive antibiotics lung disease.