Osteogenic differentiation of human ligament fibroblasts induced by conditioned medium of osteoclast-like cells

• DOI: 10.5582/bst.2011.v5.2.46
• Publication Date: 2011-05-06T13:35:41Z
• Authors: Fangcang Yu; Yazhou Cui; Xiaoyan Zhou; Xiume Zhang; Jinxiang Han
• Keywords: 0301 basic medicine; Ligaments; Osteoclasts; Cell Differentiation; Fibroblasts; Alkaline Phosphatase; MicroRNAs; 03 medical and health sciences; Calcification, Physiologic; Gene Expression Regulation; Osteogenesis; Culture Media, Conditioned; Humans; RNA, Messenger; Cells, Cultured; Oligonucleotide Array Sequence Analysis

Osteoclasts secrete factors that may promote mesenchymal stem cell mineralization in vitro. Fibroblasts are the most common cells connective tissue and involved process of exotic ossification many diseases such as ankylosing spondylitis. The purpose this study was to investigate whether osteoclast-like would induce osteogenic differentiation fibroblasts In present study, (OLCs) were generated by CD14+ from human peripheral blood. primarily cultured spinal ligaments. After treatment with conditioned medium OLCs, level alkaline phosphatase (ALP) increased significantly. cDNA microarray analysis identified a set differentially expressed mRNA associated signal transduction, differentiation, bone formation, microRNA expression profiles revealed group microRNAs, including hsa-miR-20a, hsa-miR-300, hsa-miR-185, hsa-miR-30d, hsa-miR-320a, hsa-miR-130b, hsa-miR-33a, hsa-miR-155, hsa-miR-222, significantly down-regulated. These microRNAs predicted have an inhibitory effect on genes BMP2, Osteocalcin, Runx2. current results suggest osteoclasts might vitro miRNA play important role regulation cell-cell interaction between fibroblasts.