Cordycepin induces apoptosis in human bladder cancer T24 cells through ROS-dependent inhibition of the PI3K/Akt signaling pathway

• DOI: 10.5582/bst.2019.01214
• Publication Date: 2019-09-16T22:03:30Z
• Authors: Sung Ok Kim; Hee-Jae Cha; Cheol Park; Hyesook Lee; Su Hyun Hong; Soon-Jeong Jeong; Shin-Hyung Park; Gi-Young Kim; Sun-Hee Leem; Cheng-Yun Jin; Eun-Joo Hwang; Yung Hyun Choi
• Keywords: Cordycepin; LY294002; Viability assay

Cordycepin, a derivative of nucleoside adenosine, is one the active ingredients extracted from fungi genus Cordyceps, which have been used for traditional herbal remedies. In this study, we examined effect cordycepin on proliferation and apoptosis human bladder cancer T24 cells its mechanism action. Cordycepin treatment significantly reduced cell survival rate in concentration-dependent manner, was associated with induction apoptosis. activated caspase-8 -9, are involved initiation extrinsic intrinsic pathways, respectively, also increased caspase-3 activity, typical caspase, subsequently leading to poly (ADP-ribose) polymerase cleavage. Additionally, Bax/Bcl-2 ratio truncation Bid, destroyed integrity mitochondria, contributed cytosolic release cytochrome c. Moreover, effectively inactivated phosphoinositide 3-kinase (PI3K)/Akt signaling pathway, while LY294002, PI3K/Akt inhibitor, apoptosis-inducing cordycepin. further enhanced intracellular levels reactive oxygen species (ROS), addition N-acetyl cysteine (NAC), ROS diminished cordycepin-induced mitochondrial dysfunction growth inhibition, blocked inactivation pathway. Furthermore, presence NAC attenuated apoptotic death reduction viability by LY294002. Collectively, data indicate that induces through activation pathways ROS-dependent cells.