Comparison of the extraction and determination of serum exosome and miRNA in serum and the detection of miR-27a-3p in serum exosome of ALS patients

• DOI: 10.5582/irdr.2017.01091
• Publication Date: 2018-03-14T22:43:27Z
• Authors: Qian Xu; Yuying Zhao; Xiaoyan Zhou; Jing Luan; Yazhou Cui; Jinxiang Han
• Keywords: Exosome

Amyotrophic Lateral Sclerosis (ALS) is a muscle-bone degenerative disease, which lacks specific index for diagnosis. In our previous studies, we found that exosomes mediated the interaction mechanism between muscle and bone at cellular level, myoblast can transfer miR-27a-3p to promote osteoblast mineralization. Therefore, suppose expression of in serum ALS patients also changes. this study, used healthy human as sample find out conditions methods extraction detection. Then through comparison 10 subjects, was down-regulated, may be involved development ALS, therefore has potential reference diagnosis clinic.