Isolation and characterization of Aurantiochytrium species: high docosahexaenoic acid (DHA) production by the newly isolated microalga, Aurantiochytrium sp. SD116

• DOI: 10.5650/jos.62.143
• Publication Date: 2013-03-06T07:37:58Z
• Authors: Mang Gao; Xiaojin Song; Yingang Feng; Wenli Li; Qiu Cui
• Keywords: Yeast extract

A heterotrophic microalga, strain SD116, with the ability to produce high concentrations of docosahexaenoic acid (DHA, C22:6n-3) was isolated from Shuidong Bay, Guangdong Province, China. Nucleotide sequence analysis 18S rDNA SD116 showed that has a close phylogenetic relationship Aurantiochytrium species. The highest rates for growth and DHA accumulation were obtained in 6.0% glucose, 2.0% yeast extract, 50% artificial seawater (ASW) at pH 7 28°C. maximum total lipid content reached 56.3% dry cell weight (DCW), accounted 50.9% fatty (TFA) content. It further found urea may be potential nitrogen source industrial fermentation because its cheap price induce relatively biomass production capacity. Using 5 L fermenters, DCW, content, yield 70.43 g L–1, 71.09% 17.42 L–1 (34.79% TFA), respectively. results show sp. is promising candidate commercial could useful synthesis biomass-related products.