Obstructive Apneas Induce Early Release of Mesenchymal Stem Cells into Circulating Blood

• DOI: 10.5665/sleep/32.1.117
• Publication Date: 2017-09-07T06:47:32Z
• Authors: Isaac Almendros; Josep M. Montserrat; Daniel Navajas; Ramon Farré; Alba Carreras; Irene Acerbi
• Keywords: Male; 0301 basic medicine; Sleep Apnea, Obstructive; Stem Cells; Bone Marrow Cells; Cell Count; Cell Differentiation; Mesenchymal Stem Cells; Fibroblasts; Osteocytes; Blood Cell Count; Rats; 3. Good health; Rats, Sprague-Dawley; Disease Models, Animal; 03 medical and health sciences; Adipocytes; Cell Adhesion; Animals; Cell Division

To investigate whether noninvasive application of recurrent airway obstructions induces early release of mesenchymal stem cells into the circulating blood in a rat model of obstructive sleep apnea.Prospective controlled animal study.University laboratory.Twenty male Sprague-Dawley rats (250-300 g).A specially designed nasal mask was applied to the anesthetized rats. Ten rats were subjected to a pattern of recurrent obstructive apneas (60 per hour, lasting 15 seconds each) for 5 hours. Ten anesthetized rats were used as controls.Mesenchymal stem cells from the blood and bone marrow samples were isolated and cultured to count the total number of colony-forming unit fibroblasts (CFU-F) of adherent cells after 9 days in culture. The number of CFU-F from circulating blood was significantly (P = 0.02) higher in the rats subjected to recurrent obstructive apneas (5.00 +/- 1.16; mean +/- SEM) than in controls (1.70 +/- 0.72). No significant (P = 0.54) differences were observed in CFU-F from bone marrow.Application of a pattern of airway obstructions similar to those experienced by patients with sleep apnea induced an early mobilization of mesenchymal stem cells into circulating blood.