Distinct Phase Relationships between Suprachiasmatic Molecular Rhythms, Cerebral Cortex Molecular Rhythms, and Behavioral Rhythms in Early Runner (CAST/EiJ) and Nocturnal (C57BL/6J) Mice

• DOI: 10.5665/sleep.2120
• Publication Date: 2012-09-28T15:41:00Z
• Authors: Peng Jiang; Kathleen M. Franklin; Marilyn J. Duncan; Bruce F. O'Hara; Jonathan P. Wisor
• Keywords: PER1; PER2

We have previously established that CAST/EiJ (CAST) mice differ from normal mice, such as C57BL/6J (B6), in the timing of wheel-running onset relative to light/dark cycles. These provide an animal model for studies genetic and neurobiological basis circadian phase misalignment humans. Neither differences endogenous period nor shape photic response curve explain difference activity between CAST B6 suggesting a mechanism downstream clock. Here, we further test hypothesis two strains with respect oscillations at molecular level.Sleep/wake cycles were examined rhythms Period1 (Per1) Period2 (Per2) expression measured cerebral cortex, suprachiasmatic nucleus (SCN), other hypothalamic regions.Basic sleep research laboratory.Male inbred strains.None.Sleep/wake advanced by approximately 4 h mice. This was paralleled phase-advanced Per1 Per2 expression, byin situ hybridization, cortex B6. By contrast, induction ofPer1 SCN unaffected strain.The wheel running sleep/wake is apparently not associated clock itself, but most likely mechanisms may therefore system investigate underlying unusual patterns entrainment ambient photoperiod.Jiang P; Franklin KM; Duncan MJ; O'Hara BF; Wisor JP. Distinct relationships rhythms, behavioral early runner (CAST/EiJ) nocturnal (C57BL/6J) SLEEP 2012;35(10):1385-1394.