Detection of Chikungunya Virus from Sera of Dengue-Suspected Patients in the Philippines

• DOI: 10.57043/transnastphl.2000.5404
• Publication Date: 2025-04-14T02:59:00Z
• Authors: Shingo Inoue; Ronald Matias; Futoshi Hasebe; Kouichi Morita; Kazunori Oishi; Jhoe Alfon; Jingle Candelario; Deu Cruz; Giselle Espiritu; Alma Gonzales; Mary Pancho; Cynthia Mapua; Efren Dimaano; Celia Carlos; Akira Igarashi; Filipinas Natividad

Due to the similar symptoms with dengue fever (DF) and hemorrhagic (DHF), Chikungunya (CHIK) virus infections were diagnosed as DF or DHF. In this study, we examined more than 300 serum samples collected from dengue-suspected patients in San Lazaro Hospital St. Luke's Medical Center. Reverse transcriptase-polymerase chain reaction (RT-PCR) antigen sandwich enzyme-linked immunosorbent assay (ELISA) applied for CHIK detection. IgM-indirect immunofluorescent antibody test (IgM-IFA), IgM capture ELISA, IgG indirect ELISA detection of serological evidence infection. Although could not detect any by RT-PCR, 4.1% confirmed showed high titer ELISA. By IgM-IFA, 15.3% positive virus. Thirteen point seven percent IgM-capture Eighteen five