Fisetin alleviates oxidative stress and promotes porcine early embryonic development via activation of the NRF2-ARE signalling pathway

• DOI: 10.5713/ab.24.0691
• Publication Date: 2025-02-27T04:16:31Z
• Authors: Hua-Kai Wei; Jia-Jia Qi; Yan-Qiu Wang; He-Xuan Qu; Chen-Xuan Yan; Tian-Tian Li; Yu Wang; Hao Sun; Bo-Xing Sun; Shuang Liang
• Keywords: Fisetin; Signalling

We improved the developmental capacity of porcine early embryos via supplementation with fisetin during in vitro culture (IVC). In addition, we investigated antioxidant mechanism activation NRF2-ARE signalling pathway embryos. Fisetin (0, 1, 2.5 and 5 μM) was supplemented IVC to observe its effects on ability parthenogenetic (PA), fertilization (IVF) somatic cell nuclear transfer (SCNT) The capacity, mitochondrial function, proliferation apoptosis levels PA were detected fluorescence staining, expression genes related apoptosis, pluripotency NRF2 also examined. Compared control, 1 μM increased PA, IVF SCNT Additionally, significantly decreased reactive oxygen species (ROS) levels; embryonic development, glutathione (GSH) function Moreover, Kelch-like ECH-associated protein (KEAP1) decreased, NFE2-like bZIP transcription factor 2 (NRF2) downstream enzymes after supplementation. Our data reveal that protects from oxidative stress by activating pathway, thereby improving success embryo production.