Effects of microbial enzymes on starch and hemicellulose degradation in total mixed ration silages

• DOI: 10.5713/ajas.16.0046
• Publication Date: 2016-04-22T02:15:42Z
• Authors: Tingting Ning; Huili Wang; Mingli Zheng; Dongze Niu; Sasa Zuo; Chuncheng Xu
• Keywords: Hemicellulose; Degradation

Objective This study investigated the association of enzyme-producing microbes and their enzymes with starch hemicellulose degradation during fermentation total mixed ration (TMR) silage. Methods The TMRs were prepared soybean curd residue, alfalfa hay (ATMR) or Leymus chinensis (LTMR), corn meal, vitamin-mineral supplements, salt at a ratio 25:40:30:4:0.5:0.5 on dry matter basis. Laboratory-scale bag silos randomly opened after 1, 3, 7, 14, 28, 56 days ensiling subjected to analyses quality, carbohydrates loss, microbial amylase hemicellulase activities, succession dominant amylolytic hemicellulolytic microbes, enzymatic properties. Results Both ATMR LTMR silages well preserved, low pH high lactic acid concentrations. In addition substantial loss water soluble carbohydrates, was also observed in both TMR prolonged ensiling. activity remained detectable throughout silages, whereas progressively decreased until it inactive day 14 post-ensiling silages. During early stage fermentation, main amylase-producing Bacillus amyloliquefaciens (B. amyloliquefaciens), B. cereus, licheniformis, subtilis silage flexus, Paenibacillus xylanexedens (P. xylanexedens) silage, Enterococcus faecium closely associated hydrolysis later amyloliquefaciens, pumilus, P. source respectively. Conclusion contributes process participates only Keywords: Total Mixed Ration; Starch; Hemicellulose; Degradation; Microbial Enzymes