Diagnosis of Causative Acute Childhood Viral Diarrhea by Multiplex Real-time PCR Among Children in a Referral Hospital, Tehran, Iran

• DOI: 10.5812/apid-150816
• Publication Date: 2024-09-28T09:45:12Z
• Authors: Maryam Rajabnejad; Leila Azimi; Hannan Khodaei; Abdollah Karimi
• Keywords: Kowsar; Acute diarrhea

Background: Viral gastroenteritis has significant public health implications and can greatly affect individuals' overall well-being. It spreads rapidly within communities, particularly among pediatric populations in settings like schools. Objectives: This study aimed to identify the most common viral causes of children aged one month 18 years over a year referral tertiary children's hospital, highlighting burden illness this population. Methods: In cross-sectional study, stool samples from with admitted Mofid Children's Hospital were collected January 2023 October 2024. Total RNA extraction was performed following preparation. Detection SARS-Cov-2, norovirus, sapovirus, rotavirus, adenovirus, astrovirus carried out using multiplex Real-Time PCR. Results: A total 100 participants included which 62% male 38% female, mean age 2.7 ± 3.44 years. Among all patients diarrhea, 55.5% tested positive via Rotavirus frequently detected virus, accounting for 43.6% cases, followed by Norovirus Adenovirus at 23.6%, Sapovirus Coronavirus 14.5%, Astrovirus 7.3%. Co-infection two viruses observed 23.6% patients, 1.8% co-infected three viruses. Conclusions: highlights that young decreased many countries introduction rotavirus vaccine. An important finding research is prevalence co-infection should be taken into consideration physicians.