Effects of aspirin on the expression of nuclear factor-κB in a rat model of acute pulmonary embolism

• DOI: 10.5847/wjem.j.issn.1920-8642.2014.03.013
• Publication Date: 2014-09-04T12:36:55Z
• Authors: Ling-cong Wang; Rong-lin Jiang; Wei Zhang; Li-ling Wei; Ru-hui Yang
• Keywords: Chloral hydrate

BACKGROUND: Acute pulmonary embolism (APE) is a disorder involving the circulation resulting from blockage of artery.The present study aimed to investigate effects aspirin on nuclear factor-κB (NF-κB) activity in rat model APE. METHODS:A total 108 healthy male Sprague-Dawley rats were randomly assigned into six groups (n=18 per group): control group, sham operation APE and low-, medium-and high-dose groups.Six, 24, 72 hours after induction APE, given at respective daily dose 150, 300, 600 mg/kg by gavage for three consecutive days.Rats other treated with equal volumes normal saline.Six each group anesthetized 10% chloral hydrate solution time point, then lung tissues collected analyzed using immunohistochemical staining. RESULTS:Positive staining was bronchial epithelial cells, alveolar macrophages, surrounding smooth muscle cells.When compared number positive cells signifi cantly lower point (P<0.001).Statistically cant differences also observed among aspirin-treated 6 (P<0.05,P<0.001).Compared NF-κB protein expression reduced (P<0.05,P<0.001).Rats had thrombosis, damaged walls, hemorrhage, along different degrees infl ammatory cellular infi ltration point.However, pathological changes such as hemorrhage attenuated treatment.CONCLUSION: Aspirin can inhibit dose-dependent manner, alleviate injury