Innate Immune-Related Receptors in Normal and Psoriatic Skin

• DOI: 10.5858/2003-127-178-iirrin
• Publication Date: 2021-01-29T16:45:32Z
• Authors: Jonathan L. Curry; Jian-Zhong Qin; Brian Bonish; Ryan Carrick; Patricia Bacon; Jeffrey Panella; June Robinson; Brian J. Nickoloff
• Keywords: TLR9; Epidermis (zoology)

Abstract Context.—A precise role for the innate immune system in psoriasis remains to be determined. Surface receptors, including Toll-like receptors (TLRs) that recognize bacterial ligands and CD91, which recognizes heat shock proteins (HSPs), are implicated both adaptive immunity. Objective.—Since skin is exposed various exogenous stimuli, can provoke or exacerbate psoriasis, we characterized expression function of TLRs, HSPs normal psoriatic skin. Design.—A variety skin-derived cells blood-derived were analyzed vivo vitro; samples obtained from 24 different individuals immune-related receptor function. By comparing contrasting with healthy patients, several specific differences identified. Results.—Immunohistochemistry-based profiling revealed TLR1 epidermal dendritic (DCs) dermal (DDCs) skin, as well pre-psoriatic plaques, enhanced basal layer keratinocyte (KC) plaques compared skin; TLR2 primarily by DDCs; TLR4 DCs DDCs, mid-epidermal-layer KCs displaying cell surface staining. No TLR9 CD14 was detected on KCs, although contained CD14-positive macrophages. Analysis epidermis 27, 60, 70. Keratinocytes CD91 negative, but expressed fibroblasts DDCs prominent accumulation CD91-positive plaques. Cultured no TLR2, TLR4, TLR9, CD91. Exposure fibroblasts, not lipopolysaccharide triggered nuclear factor (NF)-κB activation. Heat did induce maturation accompanied increased interleukin-12 production antigen-presenting Conclusions.—These data demonstrate distinctive patterns subsets suggesting functional roles psoriasis.