Osteogenic Differentiation of Human Amniotic Epithelial Cells and Its Application in Alveolar Defect Restoration

• DOI: 10.5966/sctm.2014-0118
• Publication Date: 2014-11-04T03:08:30Z
• Authors: Si Jiawen; Zhang Jianjun; Dai Jiewen; Yu Dedong; Yu Hongbo; Shi Jun; Wang Xudong; Steve G.F. Shen; Guo Lihe
• Keywords: Osteopontin; Amniotic epithelial cells; RUNX2

The present study investigated the detailed in vitro osteogenic differentiation process and vivo bone regenerative property of human amniotic epithelial cells (hAECs). hAECs was evaluated by biochemical staining, real-time polymerase chain reaction, immunofluorescence. Next, β-tricalcium phosphate (β-TCP) scaffolds alone or loaded with were implanted into alveolar defects rats. Micro-computed tomography evaluation histologic studies conducted. Our results validated capacity upregulation Runx2, osterix, alkaline phosphatase, collagen I, osteopontin, positive staining for osteoblasts. An epithelial-mesenchymal transformation might be involved increased expression transforming growth factor-β1. data also demonstrated that implantation on β-TCP scaffolds, not only improved regeneration direct participation, but reduced early host immune response to scaffolds. presented indicate possess proper potential a modulatory influence tissue remodeling process, making these source progenitor clinical restoration defect.