Additional file 1: Figure S1. of Multi-target chimaeric VLP as a therapeutic vaccine in a model of colorectal cancer

• DOI: 10.6084/m9.figshare.c.3852925_d1.v1
• Publication Date: 2017-01-01
• Authors: Braeden Donaldson; Farah Al-Barwani; Simon J. Pelham; Katie Young; Vernon K. Ward; Sarah Young

In vivo and in vitro Cytotoxicity Gating Strategies. strategy used for identification of target cell populations an cytotoxicity assay. (a) Doublets were excluded by comparing side scatter (SS) interval (INT) against SS height (PEAK). (b) Live cells gated as negative Near-IR Live/Dead. (c) Target identified CFSE VPD staining, giving CFSEHi, CFSELo, CFSEHi/VPD stained populations. Unstained from Gate F partially masked to improve the comparison proportions between A similar gating was (d) INT PEAK. (e) (f) left unmasked. (JPEG 752Â kb)