Glycyrrhizin inhibits LPS-induced neutrophil-like release of NETs

• DOI: 10.62347/larn2372
• Publication Date: 2024-10-31T07:15:45Z
• Authors: Zixuan Shen
• Keywords: Glycyrrhizin

To investigate the regulatory effect of glycyrrhizin (GL) on release neutrophil extracellular traps (NETs) from neutrophils in sepsis. HL-60 cells were induced to differentiate into neutrophil-like dHL-60 establish a sepsis model. Expression levels high-mobility group box 1 (HMGB1), citrullinated histone H3 (Cit-H3), and Toll-like receptor 9 (TLR9) assessed by Western blotting. Free DNA, component NETs, was quantified using fluorescence microplate reader. Cellular immunofluorescence analysis used detect expression key NETs protein, Cit-H3. stimulated with 200 ng/ml LPS exhibited highest The model showed significantly increased Cit-H3 HMGB1. GL intervention reduced HMGB1 decreased free DNA level. These findings suggest that decreases NET TLR9 elevated Exogenous recombinant human protein further expression, while inhibited this increase. may inhibit through HMGB1/TLR9 pathway.