Rapid Determination of Andrographolide and Dehydroandrographolide in <i>Andrographis paniculata</i> by HPLC-UV at Equal Absorption Wavelength

• DOI: 10.62767/jecacm502.5966
• Publication Date: 2024-06-07T02:42:39Z
• Authors: Qi Huang; Qinggui Lei; Da Li; Wenqing Li; Zhengming Qian
• Keywords: Andrographis Paniculata; Andrographolide

A rapid and reference component saving method for the determination of andrographolide dehydroandrographolide in Andrographis paniculata was developed this study. The sample prepared using matrix solid-phase dispersion analyzed by an Agilent Poroshell 120 EC-C18 column (50 mm × 4.6 mm, 2.7 μm) with a water-acetonitrile mobile phase. equal absorption wavelength two components confirmed to be 240 nm combination ultraviolet spectrophotometer high performance liquid chromatograph. Results showed that exhibited good linearity their concentration ranges (r ≥ 0.9999), limit quantification 80.85 μg/g 80.63 μg/g, respectively. Two also had precision as relative standard deviation (RSD) value less than 1.00%. average recoveries were 102.80% (RSD = 1.54%) 100.70% 1.91%), shared same result external Chinese Pharmacopoeia, which took total 10 minutes, consumed 8.5 mL harmful solvent, only used one determine content components. This is characterized low consumption study on main active its products, contributes quality evaluation paniculata.