Effect of 24-epibrassinolide on lipoxygenase activity in maize seedlings under cold stress

• DOI: 10.7124/bc.00015a
• Publication Date: 2012-12-14T19:41:08Z
• Authors: V. N. Kopich; S. V. Kretynin; O. V. Kharchenko; R. P. Litvinovskaya; N. M. Chashina; V. A. Khripach
• Keywords: 0301 basic medicine; 0303 health sciences; 03 medical and health sciences; Bioorganic Chemistry

????????. ???????????????????????? ?????????????? 24-?????????????????????????????? ???? ???????????????????? 9- ?? 13-???????????????????????? (9- ?? 13-??????) ???? ???????????????????? ???????????????? ?????? ???????????????????? ???????????????? (25 C) ?? ?????? ???????????????? ??????????-???????????????????????????? ?????????????? (5 C). ????????????. ???????????????????? ?????? ???????????????? ?????????? ?????????????????? ???????????????????? 0,01 ?? 1 ?????? 24-????????????????????????????????. ???????????????? ???????????????????????????? ???? ???????????????????? ???????????????? ?? 0,1 ?? ????????????-?????????????????? ???????????? (???? 4,5) ?? ?????????????????????? ?????????????????? ???????????????????? (0,1% Brij-99) ?? ???????? (0,1 ??M). ???????????????????? ?????? ???????????????????? ?????????????????????????????????????????? ?????? 234 ???? ?? ???????????????????????????? ?????????????????? ?????????????? ?? ???????????????? ?????????????????? (????. 6,0 ?? 7,0) ?? ?????????????????????? ?? ?? ???????????????????? 0,02 % Lubrol ???? ????????????????????????????. ????????????????????. ????????????????, ?????? ?? ???????????????????? ???????????????? ?? ???????????????????????? 24-???????????????????????????????? ???????????????????? ???????????????????? ?????? ???????????????????? ?? 3???6 ??????. ?????? ???????????????? ?????????????????????????????????????? ?????????????? ?? ?????????????????????? 1 ????M 24-?????????????????????????????? ???????????????????? 9- ?? 13-?????? ?????????????????????????? ?? 4 ?? 10 ?????? ????????????????????????????. ????????????. ???????????????????? ?? ?????????????????? ???????????? ???????????????????? ?????????????????? ???????????????????????? ???? ?????????????? ?????????????????????????? ?? ?????????????????? ???????? ???????????????????? ?????????????????????? ?????? ?? ???????????????????????? ???????????????????? ???????????? ???? ???????????????? ??????????????????????????????????.<br/>Aim. To investigate 24-epibrassinolide influence on the maize seedlings 9- and 13-lipoxygenases activity (9- and 13-LOX) under normal conditions (25 oC) and cold stress (5 oC). Methods. LOX activity was measured after treatment of seedlings with 0.01 and 1 ??M 24-epibrassinolide. The enzymes were extracted from maize seedlings with 0.1 M sodium acetate (pH 4.5) buffer, supplemented with a non-ionic detergent (0.1 % Brij-99) and EDTA (0.1 mM). The 9- and 13-LOX activities were determined spectrophotometrically at 234 nm using linoleic acid as substrate at pH 6.0 and 7.0 in the presence or absence of 0.02 % Lubrol PX. Results. 3-6-fold increase in LOX activity of 24-epibrassinolide-treated seedlings was demonstrated under normal conditions. Cold stress in the presence of 1??M 24-epibrassinolide enhances the activities of 9- and 13- LOX by 4 and 10 times, respectively. Conclusions. The results obtained enlarge our understanding of possible pathways of LOX metabolites involvement in the formation of cell response to brassinosteroids.<br/>????????. ?????????????????????? ???????????? 24-?????????????????????????????? ???? ???????????????????? 9- ?? 13-???????????????????????? (9- ?? 13-??????) ?? ???????????????????? ?????????????????? ???? ???????????????????? ???????? (25 C) ???? ???? ?????? ???????????????????????????????????????? ???????????? (5 C). ????????????. ???????????????????? ?????? ?????????????????? ???? ???????? ?????????????? ???????????????????? 0,01 ?? 1 ?????? 24-????????????????????????????????. ???????????????? ???????????????????????? ?? ???????????????????? ?????????????????? ?? 0,1 ?? ????????????-???????????????????? ???????????? (???? 4,5) ???? ?????????????????????? ?????????????????? ???????????????????? (0,1 % Brij-99) ???? ???????? (0,1 ??M). ???????????????????? 9- ?? 13-?????? ?????????????????? ?????????????????????????????????????? ?????? 234 ???? ?? ?????????????????????????? ?????????????????? ?????????????? ???? ?????????????????? ?????? ???? 6,0 ?? 7,0 ???? ?????????????????????? ???? ?????????????????????? 0,02 % Lubrol ???? ????????????????????. ????????????????????. ????????????????, ???? ???? ???????????????????? ???????? ?? ???????????????????? 24-???????????????????????????????? ???????????????????? ???????????????????? ?????? ?????????????? ?? 3???6 ??????????. ???? ?????? ???????????????????????????????????????? ???????????? ???? ?????????????????????? 1 ?????? 24-?????????????????????????????? ???????????????????? 9- ?? 13-?????? ?????????????????????????? ?? 4 ???? 10 ?????????? ????????????????????. ????????????????. ???????????????? ?? ???????????? ???????????????????? ???????????????????? ?????????????? ???? ???????????????? ???????????????? ???????? ?????????????????? ?????????? ?????????????????? ?????????????????????? ?????? ???? ???????????????????? ?????????????????? ?????????????????? ???? ?????? ??????????????????????????????????.<br/>