Transcriptional and posttranscriptional regulation of the adaptor/scaffold protein gene ITSN1

• DOI: 10.7124/bc.000921
• Publication Date: 2016-08-20T14:10:59Z
• Authors: S. V. Kropyvko; O. S. Gubar; T. A. Gryaznova; D. Ye. Morderer; D. O. Gerasymchuk; L. A. Syvak; A. N. Grabovoy; A. V. Rynditch
• Keywords: 0301 basic medicine; 0303 health sciences; 03 medical and health sciences; Genomics, Transcriptomics and Proteomics

ITSN1 adaptor/scaffold protein takes part in a variety of physiological and pathological cellular processes.It has complex expression regulation many partners.Aim.Characterization the functio ning control is important for understanding its role cell.Methods.Bioinformatic analysis, semiquantitative analysis by RTPCR, immunoprecipitation.Results.We have described ana lyzed promoter regions, detected alternatively spliced isoforms at mRNA levels different cancer specimens.Using bioinformatic servers, we identified sites miRNA binding analyzed serine, threonine tyrosine phosphorylation protein. Conclusions.We obtained new data on pathology.We also shown pos sibility tyrosine. K e y w o r d s: ITSN1,